PENURUNAN LAJU PENUAAN REPRODUKSI MENCIT JANTAN (Mus muculus.Linn) DENGAN PEMBERIAN EKSTRAK JAHE (Zingiber officinale) DALAM PAKAN

Sutyarso Sutyarso, Sumayyah Annida, M. Kanedi, Hendri Busman, Nuning Nurcahyani

Abstract


Rimpang jahe mengandung antioksidan dan bersifat kemoprotektif, oleh karena itu kami menduga dapat menurunkan laju penuaan pada sistem reproduksi. Tujuan penelitian ini, untuk mengetahui pengaruh ekstrak jahe (Zingiber officinale) terhadap penuaan reproduksi mencit jantan (Mus musculus). Penelitian ini, menggunakan 36 ekor mencit jantan (Mus musculus) berumur 12-14 bulan, dibagi tiga kelompok masingmasing 12 ekor. Kelompok 1 sebagai kontrol, kelompok 2 dan 3 diberikan 50 mg dan 100 mg ekstrak jahe/kg pelet. Pelet diberikan selama 70 hari secara ad libitum. Selanjutnya, diamati jumlah sel-sel spermatogenik, serta jumlah dan kualitas spermatozoa. Hasil penelitian menunjukan, bahwa pemberian ekstrak jahe berpengaruh terhadap sel-sel spermatogenik dan spermatozoa (P<0,01) mencit. Hasil penghitungan spermatosit preleptoten, pakhiten, dan sel spermatid lebih tinggi, demikian juga pada jumlah spermatozoa, persentase viabilitas dan motilitas, serta morfologi normal spermatozoa lebih banyak pada kelompok yang diberi ekstrak jahe dibanding kontrol (P<0,01). Disimpulkan bahwa ekstrak rimpang jahe yang diberikan pada mencit yang sedang memasuki masa penuaan dapat menghambat laju penurunan fungsi repruduksi.

Keywords


ekstrak jahe; fungsi reproduksi; mencit jantan; anti-penuaan; antioksidan

Full Text:

PDF

References


Aleissa MS. 2014. Effet of ginger supplements on some reproductive parameters and spermatogenesis of mice. Indian J Fund Appl Life Sci;4:271-77

Bhandari U, Kanojia R, Pillai KK. 2005. Effect of ethanolic extract of Zingiber officinale on dyslipidaemia in diabetic rats. J Ethnopharm;97:227-30

Billig H, Furuta I, Rivier C, Tapanainen J, Parvinen M, Hsueh AJ. 1995. Apoptosis in testis germ cells: developmental changes in gonadotropin dependence and localization to selective tubule stages. Endocrinology;136:5-12

Desai N, Sabanegh Jr E, Kim T, Agarwal A. 2010. Free Radical Theory of Aging: Implications in Male Infertility. Urology;75: 14-19

Ekaluo, U.B., Ikpeme, E.V. And Edu, N.E. 2011. Biological response of male rats to aqueous extract of ginger (Zingiber officinale Roscoe.). Int J Recent Sci Res;2:240-42.

Fabricant JD, Parkening TA. 1982. Sperm morphology and cytogenetic studies in ageing C57BL/6 mice. J Reprod Fertil;66:485-89

Franks LM, Payne J. 1970. The influence of age on reproductive capacity in C57BL mice. J Reprod Fertil;21:563-65

Furuchi T, Masuko K, Nishimune Y, Obinata M,Matsui Y. 1996. Inhibition of testicular germ cell apoptosis and differentiation in mice misexpressing Bcl-2 in spermatogonia. Development;122:1703-

George SK, Jiao Y, Bishop CE, Lu B. 2012. Oxidative stress is involved in agedependent spermatogenic damage of Immp2l mutant mice. Free Rad Bio Med;52:2223-2232 Hafez DA. 2010. Effect of extracts of ginger goots and cinnamon bark on fertility of male diabetic rats. J Am Sci;6:940-47

Hozayen WG, Soliman HA, Abou-Seif HS. 2014. Study of the chemopreventive effects of Zingiber officinale roots against aspartame induced testicular toxicity in rat model. J Phys Pharm Adv;4:360-67

Jung A, Schuppe HC, Schill WB. 2002. Comparison of semen quality in older and younger men attending an andrology clinic. Andrologia;34:116-22

Kamtchouing P, Mbongue-Fandio GY, Dimo T, Jatsa HB. 2002. Evaluation of androgenic activity of Zingiber officinale extracts in

male rats. Asian J Androl;4:299-301

Kerr JFR, Wyllie AH, Currie AR. 1972. Apoptosis: A Basic biological phenomenon with wide-ranging implications in tissue

kinetics. British J Cancer; 26:239-57

Khaki A, Fathiazad F, Nouri M, Khaki A, Chelar CO, Marefat N, Hamadeh M. 2009. The Effects of Ginger on Spermatogenesis

and Sperm parameters of Rat. Iranian J Reprod Med;7:7-12.

Khaki A, Khaki AA, Hajhosseini L, Golzar FS, Ainehchi N. 2014. The anti-oxidant effects of ginger and cinnamon on

spermatogenesis dys-function of diabettes rats. AfricanTradit Comp Altern Med;11:1-8.

Kuhnert B, Nieschlag E. Reproductive functions of the ageing male. 2004. Hum Reprod Update;10:327-39.

Lotfi N, Khazaei E, Shariatzadeh SMA, Mehranjani MS, Ghanbari A. 2013. The effect of Cannabis sativa hydroalcoholic extract

on sperm parameters and testis histology in rats. Int J Morphol;31:82-6

Maheshwari A, Misro MM, Aggarwal A, Sharma RK. Nadan D. 2011. N-Acetyl-L-cysteine counteracts oxidative stress and prevents H2O2 induced germ cell apoptosis through down-regulation of caspase-9 and JNK/c-jun. Moll Reprod Dev;78:69-79

Mahluji S, Ostadrahimi A, Mobasseri M, Attari VE, Payahoo L. 2013. Anti-Inflammatory Effects of Zingiber Officinale in Type 2 Diabetic Patients. Adv Pharm Bull;3:273-76

Mares WAAK and Najam WS. 2012. The effect of ginger on semen parameters and serum FSH, LH & testosterone of infertile men. Tikrit Medical J;18:322-29

Mascolo N, Jain R, Tain S, Capasso F. 1989. Ethnopharmacologic investigation of ginger (Zingiber officinale). J Ethno Pharmacol; 27:129–40

Meistrich ML. Effects of chemotherapy and radiotherapy on spermatogenesis. 1993. Eur Urol;23:136-41

Mitchell SJ., Longo DL., Scheibye-Knudsen M, and de Cabo R. 2015. Animal Models of Aging Research: Implications for Human Aging

and Age-Related Diseases. Ann Rev Anim BioSci;3:283–303

Morakinyo AO, Adeniyi OS, Arikawe AP. 2008. Effects of Zingiber officinale on reproductive functions in the male rat.

African J Biomed Res;11:329-34

Morakinyo AO, Achema PU, Adegoke OA. 2010. Effect of Zingiber officinale (Ginger) on sodium arsenit-induced reproductive

toxicity in male rats. African J Biomed Res;13:39-45

Morakinyo AO, Oludare GO, Aderinto OT, Tasdup A. 2011. Antioxidant and fre radical scavenging activities of aqueous and ethanol extracts of Zingiber officinale. Bio Med;3:25-30.

Paul C, Teng S, Saunders PTK. 2009. A single mild-transient scrotal heat stress causes hypoxia and oxidative stress in mouse testes which induces germ cell death. Biol Reprod;80: 913-19.

Rasyidah TI, Suhana S, Nur HA, Kaswandi MA, Noah RM. 2014. Evaluation of antioxidant activity of Zingiber officinale (ginger) on formalin-induced testicular toxicity in rats. J Med Bioeng;3:149-53 Sakr SA and Badawy GM. 2011. Effect of ginger (Zingiber officinale R.) on metiraminhibited spermatogenesis and induced apoptosis in albino mice. J App Pharm Sci;1:131-36.

Schmidt JA, Oatley JM, Brinster RL. 2009. Female Mice Delay Reproductive Aging in Males. Biol Reprod;80:1009-14.

Sinha HAP, Rajavashisth TB, SinhaHI, Lue Y, Bonavera JJ, Leung A, Wang C, Swerdloff RS. 1997. Significance of apoptosis in the temporal and stagespecific loss of germ cells in the adult rat after gonadotropin deprivation. Biol Reprod;57:1193-201

Systin P, Chen H, Zirkin BR, Robaire B. 2001. Gene expression in Brown Norway rat Leydig cells: Effect of age and agerelated germ cell loss. Endocrin;142:5277-85

Sutyarso, Muhartono, Susianti, Busman H, Kanedi M. 2016. Testicular Function of Rats Treated with Water Extract of Red Ginger (Zingiber officinale var.rubrum) Combined with Zinc. Journal of Food and Nutrition Research; 4( 3):157-162

Sutyarso, Busman H, Kanedi M, Muhartono. 2016. Rhizome Extract of White Ginger (Zingiber officinale Roxb) Maintains Testicular Function of Aging Mice. International Journal of Nutrition and Food Sciences;5(3):175-178

Sutyarso. 2017. Penuaan dan Fungsi Seksual. Orasi Ilmiah Pengukuhan Guru Besar. Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Lampung.

World Health Organization. 2010. WHO laboratory manual for the examination and processing of human semen. 5th Ed.,

Geneva, Switzerland; World Health Organization:21-65

Yang HS, Han DK, Kim JR, Sim JC. 2006. Effet of alpha-tocopherol on cadmium inducedtoxicity in rat testis and carcinogenesis. Korean Med J;21:445-51

Zahedi A, Khaki A. 2014. Recovery effect of Zingiber officinale on testis tissue after treatment with gentamicin in rats. J Med

Plant Res;8:288-91

Zancan KC, Marques MO, Petenate AJ, Meireles MA. 2002. Extraction of ginger (Zingiber officinale. Roscoe) oleoresin with CO2

and co-solvents: A study of the antioxidant action of the extracts. J

Supercri Fluids;24:57-67




DOI: http://dx.doi.org/10.23960%2Fj-bekh.v5i1.2170

Refbacks

  • There are currently no refbacks.


J-BEKH: Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati
Jurusan Biologi Fakultas MIPA Universitas Lampung
Jl. Sumantri Brojonegoro No. 1 Bandarlampung
Lampung 35145


This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.