POLYSACCHARIDE DEGRADING MICROORGANISMS IV: EFFECT OF VARIOUS FACTORS ON CELL-GROWTH AND ENZYME PRODUCTION OF A LOCAL ISOLATE-PRODUCING AMYLASE STRAIN LTE-6

Mulyono Mulyono

Abstract


Effort on alternative fuel sources like bioethanol is aimed to combat the rise due to the depletion of fossil fuels. Bioethanol can be produced from a range of starchy materials, which catalyzed by amylase. Degradation of starch into simple sugars is the key of the metabolism. Previously we had reported an local amylolytic microorganisms, namely as Bacillus strain LTE-6, which showed best amylolytic activities on solid and liquid medium composed with 1% (w/v) cassava raw starch as the single Carbon and energy source. Here we are reporting the investigation the effects of C and N source, metal ions and pH on the cells-growth and enzyme production of the strain. Measurement was performed at 8, 24, 31, 48 and 55 hours incubation. The cells-growth was monitorized by measuring optical density on 600 nm and the enzyme activity was measured by methods of Fuwa and Lowry. Investigation results showed that the strain grew best and produced amylase on medium composed with 0,5% (w/v) sugar, 0,5% (w/v) peptone, 0,5% (w/v) of Fe, and pH 6.0. In this condition it was obtained respectively, the cells-growth i.e 3.75, 1.91, 3.32, 1.45, the amylase activities i.e 10,83 U/ml, 9,8 U/ml, 9,5 U/ml, 8,7 U/ml, on 48, 24, 24, and 24 hours incubation. The results was higher than in untreated medium with OD of 1.9, amylase activity of 5.5 U/ml, and 31 hour of incubation. In other words, medium composition of the culture affected on the cells-growth and amylase production of strain LTE-6. Purification of the enzyme using obtained conditions above is going to be investigated in near future.

 

Keywords : Bioethanol, raw starch cassava, amylase, culture conditions


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